Resultsįive alginate lyases from bacteria were cloned into Pichia pastoris GS115 and the alginate lyase PDE9 was expressed at relatively high yield, activity and stability in P. This study aimed to optimize the enzymatic preparation of AOS by using bacterial alginate lyases expressed in yeast, investigate the effects of the prepared AOS on the growth performance and gut health of broiler chickens, and reveal the underlying mechanisms. However, the effects of AOS on chicken health and the underlying mechanisms are not fully understood.
Alginate oligosaccharide (AOS) holds great potential as a novel feed supplement in farm animals.
